Corrected Version Specificity of the Latex Test for Cryptococcal Antigen: a Rapid, Simple Method for Eliminating Interference Factors
نویسندگان
چکیده
منابع مشابه
Specificity of the latex test for cryptococcal antigen: a rapid, simple method for eliminating interference factors.
An enzymatic method involving a protease (pronase) for the elimination of interference factors in the latex test for cryptococcal antigen was developed and compared with dithiothreitol treatment. The two were equivalent in their ability to remove interference factors; however, the enzymatic method generally yielded higher titers. The method is simple, requires only 20 min, and makes the latex t...
متن کاملInterference by hydroxyethyl starch used for vascular filling in latex agglutination test for cryptococcal antigen.
The glucuronoxylomannan component of the Cryptococcus neoformans capsular polysaccharide confers serotype specificity, and its detection in cerebrospinal fluid or serum by the latex agglutination test is used for diagnosis. Low-molecular-weight hydroxyethyl starches can be used as an alternative to albumin for vascular filling. This study reports the occurrence of a false-positive result with t...
متن کاملExperience with the use of pronase to eliminate interference factors in the latex agglutination test for cryptococcal antigen.
Cryptococcal antigen titers of 70 cerebrospinal fluid and 57 serum specimens from patients suspected of having cryptococcosis were determined both before and after treatment with pronase. Median titers of cerebrospinal fluid specimens before and after treatment were 128 and 128, respectively; mean geometric titers of these specimens before and after treatment were 102 and 204, respectively. Med...
متن کاملanti-cryptococcal-globulin-latex production for rapid detection of cryptococcus neoformans polysaccharide antigen in cryptococcosis
cryptococcosis has become the fourth leading life-threatening opportunistic infection in patients with aids, but also occurs in non-aids patients. in view of the increasing numbers of infection during last decade in iran, use of rapid, sensitive and specific test for diagnosis of cryptococcal disease has become important than ever. we aimed to produce the reagents for latex cryptococcal antigen...
متن کاملdevelopment of a latex agglutination test as a simple and rapid method for diagnosis of trichomonas vaginalis infection
trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. in this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. trichomonas vaginalis was harvested from tyis33 culture medium and anti trichomonas vaginalis antiserum ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1983
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.17.5.945-947.1983